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restriction enzymes drai, ecorv, pvuii, stui, ehei, kspai, smai, smii, afei  (Thermo Fisher)


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    Thermo Fisher restriction enzymes drai, ecorv, pvuii, stui, ehei, kspai, smai, smii, afei
    Restriction Enzymes Drai, Ecorv, Pvuii, Stui, Ehei, Kspai, Smai, Smii, Afei, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/restriction+enzymes+ehei/restriction+enzymes+drai++ecorv++pvuii++stui++ehei++kspai++smai++smii++afei/pm31529521-222-19-20
    Average 90 stars, based on 1 article reviews
    restriction enzymes drai, ecorv, pvuii, stui, ehei, kspai, smai, smii, afei - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Generation of a gene-corrected isogenic control cell line from an Alzheimer's disease patient iPSC line carrying a A79V mutation in PSEN1.
    Article Snippet: The single-guide RNAs (sgRNA) were ordered from Sigma and the cloning of CRISPRs followed the protocol from Ran et al. (2013), with a single plasmid containing both the sgRNA and the Cas9 (pSpCas9(BB)-2A-Puro (PX459) V2.0; addgene ID 48139). ssODNs were designed to contain 120 bp in total, with 60 bp upstream and 60 bp downstream of the cutting site. .. Screening of clones was performed with restriction digestions where the wild-type sequence was recognized (and cut) by specific restriction enzymes (EheI, 5′-GGC↓GCC-3′, ThermoScientific), while the mutated sequence was not, which means EheI only digested clones where the ssODN was successfully integrated. .. A79V-hiPSCs growing on matrigel-coated (Corning Bioscience) dishes with E8 medium (Gibco) were detached with Accutase (Gibco).

    Sequencing:

    Article Title: Generation of a gene-corrected isogenic control cell line from an Alzheimer's disease patient iPSC line carrying a A79V mutation in PSEN1.
    Article Snippet: The single-guide RNAs (sgRNA) were ordered from Sigma and the cloning of CRISPRs followed the protocol from Ran et al. (2013), with a single plasmid containing both the sgRNA and the Cas9 (pSpCas9(BB)-2A-Puro (PX459) V2.0; addgene ID 48139). ssODNs were designed to contain 120 bp in total, with 60 bp upstream and 60 bp downstream of the cutting site. .. Screening of clones was performed with restriction digestions where the wild-type sequence was recognized (and cut) by specific restriction enzymes (EheI, 5′-GGC↓GCC-3′, ThermoScientific), while the mutated sequence was not, which means EheI only digested clones where the ssODN was successfully integrated. .. A79V-hiPSCs growing on matrigel-coated (Corning Bioscience) dishes with E8 medium (Gibco) were detached with Accutase (Gibco).



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    Summary of the restriction analysis of phage S144 DNA, propagated on C. sakazakii CS1, S. Infantis S15 and S. Muenster S394. DNA from phage Lambda (methylated and unmethylated) have been used as controls. The restriction site for each of the nine used enzymes is specified and in each cell, it is indicated if the DNA was cut (1, in orange) or not (0, in white). SspI and PacI, the only two enzymes able to cut S144 DNA, are in bold. RE, restriction enzymes; RS, restriction sites, met., methylated; unmet., unmethylated.

    Journal: International Journal of Molecular Sciences

    Article Title: Phage S144, a New Polyvalent Phage Infecting Salmonella spp. and Cronobacter sakazakii

    doi: 10.3390/ijms21155196

    Figure Lengend Snippet: Summary of the restriction analysis of phage S144 DNA, propagated on C. sakazakii CS1, S. Infantis S15 and S. Muenster S394. DNA from phage Lambda (methylated and unmethylated) have been used as controls. The restriction site for each of the nine used enzymes is specified and in each cell, it is indicated if the DNA was cut (1, in orange) or not (0, in white). SspI and PacI, the only two enzymes able to cut S144 DNA, are in bold. RE, restriction enzymes; RS, restriction sites, met., methylated; unmet., unmethylated.

    Article Snippet: S144 DNA was digested with the FastDigest restriction enzymes EheI, MauBI, PacI, Cfr42I, XhoI, NotI, Bsp120I, SspI and SspDI (respectively, FD0443, FD2084, FD2204, ER0201, FD0694, FD0596, FD0134, FD0774, FD0774 and ER2191 from Thermo Fisher).

    Techniques: Methylation